Picro-Sirius Red Stain Kit (Cardiac Muscle)

The Picro-Sirius Red Stain Kit (For Cardiac Muscle) is intended for use in the histological visualization of thin septa and collagen fibers. This modification of the Picro-Sirius Red stain eliminates the yellow
cytoplasmic staining that can obscure thin collagenous septa. Using this procedure easily allows viewing of collagenous septa as thin as 0.2-0.5 microns. The PSR stain may be viewed using standard light
microscopy or polarized light resulting in birefringence of the  collagen fibers to distinguish between type I and type III.

Light Microscopy

Collagen: Red

Septa: Red

Cytoplasm: Colorless to Slightly Yellow

Polarized Light Microscopy

Collagen Fibers: Yellow-Orange and Green Birefringence