Movat Pentachrome Stain Kit (Modified Russell-Movat), Volume Optimized
SKU: SS1037-VO
The Movat Pentachrome Stain Kit (Modified Movat) is intended for use in histological demonstration of collagen, elastin, muscle, mucin and fibrin in tissue sections. This procedure is particularly useful when studying the heart, blood vessels and various vascular diseases.
Elastic Fibers: Black to Blue/Black Nuclei: Blue/Black Collagen: Yellow Reticular Fibers: Yellow Mucin: Bright Blue Fibrin: Bright Red Muscle: Red
Preparation of Reagents Prior to Beginning:
1. Prepare working Elastic Stain Solution by mixing:
30ml Hematoxylin Solution (5%) (mixed solution may be used for 24 hours)
15ml Ferric Chloride Solution (10%)
15ml Lugol’s Iodine Solution. 2. Note: Lugol’s Iodine Solution will cause staining of all kit vials and labels over time. This does not adversely affect the performance of this product and is merely cosmetic in nature. 3. Note: Removal of mercury deposits is not required for tissues that have been fixed in mercury containing fixatives since it will be removed by the staining solution.
Procedure (Standard):1. Deparaffinize sections if necessary and hydrate to distilled water. 2. Stain tissue section with working Elastic Stain Solution for 20 minutes. 3. Rinse in running tap water until no excess stain remains on slide. 4. Dip slide in Ferric Chloride (2%) Differentiating Solution 15-20 times and rinse in tap water. 5. Check slides microscopically for proper differentiation. Repeat step 4 if required. 6. Rinse in 2 changes of distilled water. 7. Place slide in Sodium Thiosulfate Solution (5%) and incubate for 1 minute. 8. Rinse in tap water for 2 minutes followed by 2 changes in distilled water. 9. Place slide in Acetic Acid Solution (3%) and incubate for 2 minutes to equilibrate tissue prior to staining with Alcian Blue Solution, pH 2.5. 10. Without rinsing, place slide in Alcian Blue Solution, pH 2.5 and incubate for 25 minutes. 11. Rinse in tap water for 2 minutes followed by 2 changes in distilled water. 12. Place slide in Biebrich Scarlet – Acid Fuchsin Solution and incubate for 3 minutes. 13. Rinse slide in 2 changes of distilled water. 14. Place slide in Acetic Acid Solution (1%) for 5-10 seconds with agitation. 15. Rinse quickly in distilled water. 16. Differentiate slide in 2 changes of Phosphotungstic Acid Solution (5%) for 3 minutes each. 17. Check slides microscopically for proper differentiation. Collagen should be clear but elastic fibers should still be stained. Repeat step 15 if required. 18. Rinse slide briefly in distilled water. 19. Dip slide several times (3-5) in Acetic Acid Solution (1%). 20. Shake off excess Acetic Acid Solution (1%) and without rinsing apply Tartrazine Solution and incubate for 2 minutes. 21. Rinse slide in 3 changes of absolute alcohol. 22. Clear, and mount in synthetic resin.
Preparation of Reagents Prior to Beginning:
30ml Hematoxylin Solution (5%) (mixed solution may be used for 24 hours)
15ml Ferric Chloride Solution (10%)
15ml Lugol’s Iodine Solution.
Procedure (Standard):
Applicable Placards and Hazard Symbols
Hover over each placard to see a description of its meaning
UN1170 Ethanol Solutions, 3, II
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