Eosinophil - Mast Cell Stain Kit, Volume Optimized

SKU: SS1035-VO
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SS1035-VO

The Eosinophil - Mast Cell Stain Kit is intended for simultaneous visualization of eosinophils and mast cells.

Mast Cells: Bright Blue
Eosinophils: Bright Red
Nuclei: Blue



Procedure (Standard):
  • 1. Deparaffinize sections if necessary and hydrate to distilled water.
  • 2. Stain tissue section with Astra Blue Solution for 30 minutes with frequent agitation.
  • 3. Rinse quickly in 2 changes of distilled water.
  • 4. Stain tissue section with Vital New Red Solution for 30 minutes with frequent agitation.
  • 5. Rinse quickly in 2 changes of distilled water.
  • 6. Counterstain in Hematoxylin, Mayer’s (Lillie’s Modification) for 15-30 seconds.
  • 7. Rinse slide in running tap water for 2 minutes followed by one dip in distilled water.
  • 8. Dehydrate quickly through 3 changes of absolute alcohol.
  • 9. Clear, and mount in synthetic resin.
  • Applicable Placards

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