[XL-Cal® (Immuno) Bone Decalcifier
• 1. Small biopsies, such as bone marrow, should be fixed in B-5 Sub™ for at least 3 hours prior to processing. When using 10% NBF, fix for 8-12 hours. Over fixation is not a problem; however, tissues should generally not be fixed longer than 1-2 weeks.
• 2. Place bone core biopsies in XL-Cal ® Immuno: 11-gauge biopsy for 1 hour; 8-gauge biopsy for 90-120 minutes, 2-3mm slices of bone for 3-4 hours.
• 3. Wash decalcified bone cores or tissue blocks for 10 minutes in running tap water.
• 4. Place the washed tissues on tissue processor for routine processing and infiltration with paraffin
Hover over each placard to see a description of its meaningUN3264 Corrosive Liquid, Acidic, Inorganic, n.o.s. (Nitric Acid), 8, II