Oil Red O Stain Kit (For Fat) is intended for use in the histological visualization of fat cells and neutral fat. This kit may be used ONLY on frozen tissue sections, fresh smears, or touch preps as xylenes and alcohols will dissolve fat deposits.Results:Fat Cells: RedNeutral Fat: RedNuclei: Blue
Procedure (Standard): NOTE: Heat Oil Red O Solution to 60 ° C prior to beginning. 1. Prepare fresh or frozen tissue section as usual.2. Place slide in room temperature Propylene Glycol for 5 minutes.3. Incubate slide in heated (60 ° C) Oil Red O Solution for 6-10 minutes or overnight at room temperature.Note: Prepare mixture of 85% Propylene Glycol in distilled water. 4. Differentiate tissue section in 85% Propylene Glycol for 1 minute.5. Rinse slide in 2 changes of distilled water.6. Stain tissue section with Hematoxylin, Mayer’s (Lillie’s Modification) for 1-2 minutes.7. Rinse slide thoroughly in tap water.8. Rinse slide in 2 changes of distilled water.9. Coverslip using an aqueous mounting medium.