Oil Red O Stain Kit (For Fat), Volume Optimized

SKU: SS1048-VO
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Oil Red O Stain Kit (For Fat) is intended for use in the histological visualization of fat cells and neutral fat. This kit may be used ONLY on frozen tissue sections, fresh smears, or touch preps as xylenes and alcohols will dissolve fat deposits.

  • Fat Cells: Red
  • Neutral Fat: Red
  • Nuclei: Blue

  • Procedure (Standard): NOTE: Heat Oil Red O Solution to 60 ° C prior to beginning.

  • 1. Prepare fresh or frozen tissue section as usual.
  • 2. Place slide in room temperature Propylene Glycol for 5 minutes.
  • 3. Incubate slide in heated (60 ° C) Oil Red O Solution for 6-10 minutes or overnight at room temperature.
  • Note: Prepare mixture of 85% Propylene Glycol in distilled water.
  • 4. Differentiate tissue section in 85% Propylene Glycol for 1 minute.
  • 5. Rinse slide in 2 changes of distilled water.
  • 6. Stain tissue section with Hematoxylin, Mayer’s (Lillie’s Modification) for 1-2 minutes.
  • 7. Rinse slide thoroughly in tap water.
  • 8. Rinse slide in 2 changes of distilled water.
  • 9. Coverslip using an aqueous mounting medium.
  • Applicable Placards and Hazard Symbols

    Hover over each placard to see a description of its meaning

    Use the links to download available SDS, IFU or Flyer Documents

    Note: please try the mirror link if you receive an error

    Download SDS
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    Download IFU
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