Saccomanno Fixative Saccomanno’s Cytology Fixative is a widely used fixative in cytology for specimens such as FNA’S, urine, sputums, bronchial washings, pleural and peritoneal fluids, and sputum preparations. Saccomanno’s Cytology Fixative’s low alcohol content partially fixes the specimen’s cells to protect the cellular integrity, making it a great pre-fixative for cytology specimens that must be transported or held for some time. The Saccomanno Method for sputum separates mucus from the specimen cells so the cells can be evenly spread onto a microscope slide. In the Saccomanno Method for sputum, the sputum is placed into the Saccomanno’s Fixative and then the sputum is homogenized in a micro-blender where the cells of the sputum are forced into the tip of the centrifuge tube while the mucus stays in suspension. The supernatant with the mucus is then poured out and the cells are collected to make a smear.